Oxidative Stress and Antioxidant Defense in Patients With Jaundice

Abstract

Background: The objective of our study is to measure serum Malondialdehyde level (MDA), an
index of oxidative stress, and Vitamin E level, a protective agent against oxidative stress, in Jaundice patients and study the possible correlation between them. Materials and Methods: 80 patients with jaundice (38 female and 42 male) and 50 healthy control (23 female and 27 male) were enrolled in this study. About 10 ml of venous blood were obtained from the cubital vein using disposable needles and syringes. The thiobarbituric acid method was used to measure the Malondialdehyde (MDA) which reacted with thiobarbituric acid(TBA) to give pink color that was read at (535 nm). The concentration of vitamin E in serum was determined according to a modified Hashim and Schuttringer method.
Results: A significant increase (P<0.0001) in serum Malondialdehyde (MDA) in jaundice patients as compared to that of normal healthy controls was observed. A significant decrease (P<0.0001) in serum vitamin E in jaundice patients as compared to that of normal healthy controls has been noticed. No significant correlation between serum Malondialdehyde (MDA) and serum Vitamin E in jaundice patients was observed.
Conclusion: our data shows that antioxidant capacity decreases with an increase in oxidative stress in Jaundice patients but there is no significant correlation between them.
Abbreviations: The abbreviations used are: dL, deciliter; MDA, Malondialdehyde; µl, microliter; r, Correlation factor; rpm, Round per minute; TCA, Trichloroaceticacid; TBA, thiobarbituric acid;
ROS, Reactive oxygen species.