Study the expression of Neogene TIGD3 that derived from DNA transposons in colorectal cancer cell lines

Abstract

Abstract
The process of molecular domestication that occurred on DNA transposons and give what is called neogenes that may play an important role in the human genetic instability. One of these Neogene is TIGD3 (Tigger-derived (TIGD) family of proteins) which is of unclear role in human genome.
Aim: Study the expression of Neogene TIGD3 in colorectal cancer cell lines and its possible role in carcinogenesis.
Method: By western blot method we study the protein expression of TIGD3 gene in twelve colorectal cancer cell lines (HCT116, SW48, LOVO, DLD1) which are microsatellite instable MSI, (SW480, SW620, HT29, LS123, COLO205, T84, SW403, SW1463) which are microsatellite stable MSS and in healthy tissue of colon as a control in our study.
Results: We obtained a protein expression of TIGD3 gene in all these 12 colorectal cancer cell lines with variable degree of expression with multiple isoforms which was not seen in healthy colon tissue.
Conclusion: TIGD3 may have a role in colorectal cancer either in initiation, promotion or progression which need further research and confirmation.