Phage .typing of staphylococcus aureus using locally produced phages

Abstract

Out of 60 specimens, 30 staph aureus isolates were obtained from different source location, surgical theater (instrument, walls floor and masks), nurses and in patients in S.T.H
Ten Isolates from each source of the total specimens were able to produce phage lysates after induction by heat method and detected by spotting method
By application of the spotting method it has been found that these phage lysates were able to be classified into 3 groups according to their sources:
Phage lysates from isolates 1 and 6 represent phage group A which were originally from isolates from surgical theater
Phage lysates from isolates 15 and 16 represent phage group B which were originally from isolates from Nurses.
Phage lysates from isolates 23 and 26 represent phage group C which were originally from isolates from Inpatients.
Objective and background: The main objective of this study was to produce local phage
groups from local isolates of staph aureus to be used for epidemiological purposes staphylococcus aureus is widely distributed in our environment and can cause a wide variety of infections hence phage typing is an important method to trace the sauce of such infections
Methodology: Each specimens collected as swabs from the sources was subjected to well
known established microbiological method for isolation and identification of staph aureus All isolates were tested for the presence of phage employing heat method and detected by spotting method which based on resistance on sensitivity of each isolate to a given phage lysate
Results: Thirty staph aureus were obtained from 60 specimen collected from surgical theater (instrument, walls, floor, and masks), nurses and Inpatients.
The collected isolates were distributed into 3 groups 1, 2 and 3 according to their source Spotting method revealed that group A phage lysate originally from isalatn1and 6 were able to lyse all group isolates except 1 and 6 and those in other groups which were unlysed.
Group B phage which were originally from isolates 15 and 16 was able to lyse all group 2 isolates except 15and 16 and the remaining isolates in other groups which were unlysed.
Group C phage which was originally from isolates 23 and 26 was able to lyse all group 3 isolates except 23and 26 and the remaining isolates in other groups.
Conclusion: It is concluded that 3 local phage groups of staph aureus could be applied in phage-typing of staph oureus for epidemiological purposes to trace the source of an epidemic caused by staph.aurous