Evaluation and Comparison of different clinical techniques for detection of local isolate of Salmonella enterica serovar typhi in patients with typhoid fever

Authors

  • Rasmyia Abd Abu-Resha (PhD) * Baghdad University / Science College / Dep. of Biology
  • Hussein Hafidh Abbas (MSC) ** **Al-Yarmouk University College / Dept. of Medical Laboratories Techniques

Keywords:

Typhoid fever, S. typhi, Widal test and Blood culture, PCR

Abstract

This study was carried out to evaluate and compare of three clinical techniques which included (Serology-Hematology tests (Widal and WBC count tests), Blood culture, and Polymerase Chain Reaction "PCR") for detection of Salmonella enterica serovar typhi (S. typhi) from fresh blood specimen of suspected patients with typhoid fever.
The positive results for detection of S. typhi, that were obtained from these techniques were showed in 192 case (75%), 124 case (48.4%), and 117 case (46.1%) for PCR, Blood culture, and serology-hematology tests respectively from total 254 case of suspected patients infect with typhoid fever were collected in the period of February-June 2011from Al-Kadhimiya Teaching hospital.
When evaluated of this techniques were positive employed (n = 232) , was show high sensitivity (82.8%) of PCR with higher significant (P < 0.01) while blood culture and Widal test have only (53.45, and 50.43% of sensitivity respectively).

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Published

2026-05-04

How to Cite

Rasmyia Abd Abu-Resha (PhD), & Hussein Hafidh Abbas (MSC) **. (2026). Evaluation and Comparison of different clinical techniques for detection of local isolate of Salmonella enterica serovar typhi in patients with typhoid fever. AL-Yarmouk Journal, (1), 88–102. Retrieved from https://journal.al-yarmok.edu.iq/index.php/alyj/article/view/1734

Issue

No. 1 (2014)

Section

Articles