Effect of extract of punica granatum on experimentally induced salmonellosis

Abstract

minimal inhibitory concentration (MIC) of Kirby-Baur method was need to determine the activity of medicinal plants on induced salmonellosis in rabbits. The results revealed that aqueous extract of punica granatum had anti salmonella activity with MIC (250 µg/ml) when used at stock concentration of (2000 µg/ml). At the mean time a significant potentiation was obtained by double combination of chloramphenicol with punicagranatum MIC (31.25 µg/ml), on the other hand the triplecombination of chloramphenicol plus amoxicillin and punica granatum was more-evident with MIC (7.81µg/ml). The anti salmonella activity of puni ca granatum with antimicrobial drugs when used invivo in rabbits produced a significant relief to all the symptoms corre spording with laboratory findings which was equal to 100% after 2 weeks of therapy. The postmortem of rabbits had shown a prevention of organ complications which had been reduced from 66% for untreated (Group B) to 5% for treated (Group D&E)