طريقة جديدة للتقدير الطيفي للارجنين والتربتوفان في مختلف المستحضرات الصيدلانية و في الشكل النقي باستخدام تقنية الحقن الجرياني المستمر - اندماج المناطق

Abstract

An accurate and simple CFIA-zones Merging method for determination of two different amino acids (L-arginine & tryptophan ) by using two different reactions.
The procedure for the estimation of arginine is based on the oxidation condensation reaction of arginine with a-naphthol in the presence of sodium hypobromite as an oxidizing agent in alkaline medium to produce a red colored product measured at max 501nm. While tryptophan procedure based on azo-coupling reaction of tryptophan with an azotized product which produced by treated of diphenyl amine sulphonate with sodium nitrate in acidic medium to yield a pink colored product measured at Amax 522 nm.
The ideal condition for the formation of the complexes was investigated and it was observed that the calibration curves were obeys with Beer's low at the range 3-1400µg.ml and 5-100 µg.ml¹ with detection limit 1 µg.ml¹& µg.ml for L-arginine 1 1 and tryptophan respectively.
CFIA-Merging zones system was able to estimate L-arginine and tryptophan with sampling rate 45&52 sample /h respectively.
and The novel method was found to be appropriate for the estimation of L-arginine tryptophan in pharmaceutical formulations and in it's pure form.