The Effect of two different etching modalities (EDTA and Phosphoric Acid ) on texture of enamel and dentin

Abstract

The purpose of this study was to investigate enamel and dentin surfaces that treated with different etching ethylynediaminetetraacetic modalities -acid conditioning (EDTA) and phosphoric acid conditioning using scanning electron microscop (X1000,X4000).
Method and materials: Twelve sound human upper first premolar teeth, recently extracted for orthodontic purpose were selected. The occlusal surfaces of the teeth were sectioned horizontally so that the first layer of dentin was obtained. Then the teeth were stored in an incubator at 37°C for one hour. They were randomly divided into 3 groups, (each group containing 4 teeth), and categorized as following:
Sample 1: used as a control without etching
Sample 2: enamel and dentin were etched with phosphoric acid gel 37% for15 sec.
Sample 3: enamel and dentin were
etched with EDTA 24% gel for 1 minutes. The specimens were gold sputter coated using an ion coating (E 5000 M S.E.M coater) and fixed with double layer adhesion on the stage. Then the dentin surface of all specimens were examined with a Scanning Electron Microscope SEM (Phillips SEM 510).
Result: There was no differences
between the two etching modalities for dentin etching. While phosphoric acid was superior for enamel etching, Conclusion: Although the result shows
that EDTA was superior for enamel etch and there was no difference between the two modalities for dentine etch, more studies have been suggested with higher magnification (x10000) to see collagen fiber.