Separation and purification of amylase enzyme from the sera of breast cancer patients

Abstract

Amylase was purified from breast cancer patients using ion exchange chromatography using the DEAE cellulose gel filtration technique, using a Sephadex G100 column, and using a buffer solution of (Tris-HCL) with pH (7.2) to separate the enzyme.
The results showed an increase in amylase enzyme activity, which is directly proportional to the increase in substrate concentration. The resulting shape was straight, and the Michaelis-Menten KM value was (0.363) mm). The optimum temperature for enzyme action is (37) C. The highest activity of the enzyme after incubation of the enzyme reaction mixture for (15) minutes. The results showed that the molecular weight of the enzyme purified by electrophoresis reached 60 KD.