Oxidative Stress and Antioxidant Defense in Patients With Jaundice

أجهاد التأكسد و الدفاع المضاد للتأكسد في مرضى اليرقان

Abstract

Background: The objective of our study is to measure serum Malondialdehyde level (MDA), an
index of oxidative stress, and Vitamin E level, a protective agent against oxidative stress, in
Jaundice patients and study the possible correlation between them . Materials and Methods:
80 patients with jaundice ( 38 female and 42 male) and 50 healthy control (23 female and 27
male) were enrolled in this study. About 10 ml of venous blood were obtained from the cubital
vein using disposable needles and syringes. The thiobarbituric acid method was used to
measure the Malondialdehyde (MDA) which reacted with thiobarbituric acid(TBA) to give pink
color that was read at (535 nm). The concentration of vitamin E in serum was determined
according to a modified Hashim and Schuttringer method.
Results: A significant increase (P<0.0001) in serum Malondialdehyde (MDA) in jaundice
patients as compared to that of normal healthy controls was observed. A significant decrease
(P<0.0001) in serum vitamin E in jaundice patients as compared to that of normal healthy
controls has been noticed. No significant correlation between serum Malondialdehyde (MDA) and
serum Vitamin E in jaundice patients was observed.
Conclusion: our data shows that antioxidant capacity decreases with an increase in oxidative
stress in Jaundice patients but there is no significant correlation between them .
Abbreviations: The abbreviations used are: dL, deciliter ; MDA, Malondialdehyde ; μl, microliter;
r, Correlation factor ; rpm, Round per minute; TCA, Trichloroaceticacid ; TBA, thiobarbituric acid ;
ROS, Reactive oxygen species.

يهدف هذا البحث الى قياس مستوى مالون ثنائي الالديهايد(MDA), كمؤشر الى الاجهاد التأكسدي, و مستوى فيتامين E,و هو عامل وقائي ضد الاجهاد التاكسدي , في مصول مرضى اليرقان وتقصي امكانية وجود ارتباط بينهما